Aras Part Medical International Press International Journal of Medical Parasitology and Epidemiology Sciences 2766-6492 6 3 2025 09 29 Activity of a Novel 2-Phenoxy Nicotinic Acid Hydrazide sulfonamide against Leishmania tropica 77 82 10.34172/ijmpes.6194 EN Ali Fattahi Bafghi https://orcid.org/0000-0001-8469-2187 Neda Khanizadeh https://orcid.org/0009-0004-1715-5807 Alireza Moradi https://orcid.org/0000-0001-6094-3674 Arefeh Dehghani Journal Article 10.34172/ijmpes.6194 2025 01 19 2025 06 13 Introduction: Cutaneous Leishmaniasis is an endemic health problem worldwide. The absence of an alternative chemotherapeutic approach to treating Leishmania infection requires urgent attention. So, the laboratory-trial study aimed to determine the activity of a novel 2-Phenoxy Nicotinic Acid Hydrazide sulfonamide against Leishmania tropica. Methods: The different densities (0.2, 1, 5, 25, and 125 μg /ml) of 2-PNAHS and Glucantime (0.2, 1, 5, 25, and 125 μg /ml) were provided and added to parasite cultures. Then, the anti-Leishmania activity of the 2-PNAHS against Glucantime was determined using an in-cell proliferation ELISA, BRDU (Chemiluminescent). Results: According to the results, a dose-dependent decrease was observed in the viability of the Leishmania (L) tropica promastigotes using Glucantime (P<0.05). Administration of different levels of the 2-PNAHS (0.2, 1, 5, 25, and 125 μg /ml) significantly decreased the Leishmania (L) tropica promastigotes (P<0.05). Additionally, a difference was detected in the anti-Leishmania activity of the 2-PNAHS compared to Glucantime and the control group (P<0.05). Conclusion: These results suggest that 2-PNAHS had anti-Leishmania activity better than Glucantime.