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Int J Med Parasitol Epidemiol Sci. 2021;2(3): 60-64.
doi: 10.34172/ijmpes.2021.18
  Abstract View: 1239
  PDF Download: 574

Original Article

PfLDH Detected in Etawa Crossbred Goats Using Polymerase Chain Reaction Methods

Didik Sumanto 1* ORCID logo, Sayono Sayono 1 ORCID logo, Martini Martini 2 ORCID logo, Wahyu Handoyo 1,3, Tri Dewi Kristini 1,4

1 Department of Epidemiology, Faculty of Public Health, Universitas Muhammadiyah Semarang, Indonesia
2 Faculty of Public Health, Universitas Diponegoro, Semarang, Indonesia
3 Public Health Center of Semarang, Central Java Province, Indonesia
4 Health Office of Central Java Province, Indonesia
*Corresponding Author: *Corresponding Author: Didik Sumanto, Email: , Email: didik.24272@gmail.com

Abstract

Introduction: Livestock is the potential source of blood feed for Anopheles mosquitoes. Infectious Anopheles bites enter h-Plasmodium sporozoites in the body of livestock. In the erythrocytic phase, the parasite produces several enzymes, one of which is parasite lactate dehydrogenase (pLDH). The parasite needs this enzyme to help the glycolysis process of pyruvate into lactic acid.

Methods: In this cross-sectional study, the presence of pLDH in blood specimens of Etawa crossbreed goats was explored. Samples of the stored biological material in the form of dried blood in Whatman 1001 125 filter paper included 97 specimens. PLDH was detected using the PCR method.

Results: One sample previously reported to contain P. falciparum trophozoites under microscopic examination showed positive results in PfLDH testing.

Conclusion: The parasite of P. falciparum was found capable of living up to the erythrocytic phase and forming PfLDH in the Etawa crossbreeds.

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Submitted: 11 Apr 2021
Accepted: 13 Sep 2021
ePublished: 01 Oct 2021
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