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Int J Med Parasitol Epidemiol Sci. 2020;1(3): 49-53.
doi: 10.34172/ijmpes.2020.17
  Abstract View: 1188
  PDF Download: 852

Original Article

Assessment of Cryptosporidium in Patients With Gastroenteritis by Modified Ziehl-Neelsen Staining Method in East Azerbaijan Province of Iran During 2018-2019

Alireza Javidmehr 1 ORCID logo, Yagoob Garedaghi 2* ORCID logo, Amir Babak Sioufi 3

1 Department of Food and Agriculture, Sacramento, California, USA
2 Department of Parasitology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 Department of Pathobiology, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: *Corresponding Author: Yagoob Garedaghi, Email: , Email: Yagoob.garedaghi@gmail.com, Y_garedaghi@iaut.ac.ir

Abstract

Introduction: Cryptosporidiosis is a worldwide zoonotic disease that is caused by Cryptosporidium species and leads to acute or chronic diarrhea and vomiting in patients. Due to the suitable conditions of East Azerbaijan province for parasite transmission, the present study was performed to evaluate the prevalence of Cryptosporidium infection in patients with gastroenteritis referred to hospitals and reference laboratories of East Azerbaijan province during 2018-2019.

Methods: A descriptive cross-sectional study was performed on patients with diarrhea who referred to hospitals and reference laboratories of East Azerbaijan province during 2018-2019. In this study, 180 fecal samples were collected from patients with gastroenteritis by cluster-random sampling. Stool samples were stored in 10% formalin and transferred to a parasitology laboratory and examined for parasite contamination by modified acid-fast staining (Modified Ziehl-Neelsen) method. Data were analyzed by chi-square test using SPSS.

Results: Out of 180 samples, the highest number of diarrhea samples belonged to the age group over 40 years (35.5%) and the lowest belonged to the age group of 10-20 years (7.7%). Additionally, 56% of subjects were male and 44% were female. Of these patients, 57.7% lived in urban areas and 42.3% in rural areas. Cryptosporidium was observed in 1.6% (3 cases) of patients with gastroenteritis. There was a statistically significant relationship between Cryptosporidium infection and the age of patients with diarrhea (P<0.05).

Conclusion: In the present study, a lower prevalence of cryptosporidiosis has been determined in comparison with previous studies in East Azerbaijan province. However, Cryptosporidium and other parasitic infections in the feces of patients with diarrhea referred to hospitals or reference laboratories need to be carefully diagnosed by appropriate parasitological methods.

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Submitted: 22 Jun 2020
Accepted: 30 Aug 2020
ePublished: 14 Oct 2020
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